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| Registros recuperados: 16 | |
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| Peruzzi, Stefano; Wiborg-dahle, Stine; Haffray, Pierrick; Chatain, Beatrice; Primicerio, Raul. |
| A method of geometric morphometrics was used to characterize body shape variation in meiotic gynogenetic and triploid sea bass in comparison with diploid controls. A total of 13 coordinate landmark data were recorded on the profile of 34-month-old control (n = 81), gynogenetic (n = 117) and triploid (n = 131) siblings generated from two females (A and B). Three main axes of morphometric variation were consistent, and these accounted for 60.8 and 57.6% of the total variability in females A and B, respectively. There were significant shape deviations of triploid and gynogenetic fish relative to the controls, and differences were particularly evident in the progeny of one female. Overall, triploids displayed a more slender shape than diploids, whereas... |
| Tipo: Text |
Palavras-chave: Body shape variability; Morphometry; Geometry of Procrustes distance; Triploidy; Gynogenesis; European sea bass; Dicentrarchus labrax. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00025/13651/10757.pdf |
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| Normand, Julien; Le Pennec, Marcel; Boudry, Pierre. |
| We compared the temporal dynamics of gametogenesis in diploid and triploid Pacific oysters (Crassostrea gigas) using histology. Oysters were reared in an estuarine farming site in Brittany over 3 years and their gametogenesis was monitored over the exceptionally hot summer of 2003. Both diploids and triploids showed active gametogenesis, but a high proportion of triploids remained at early stages of gonad development. Gametogenesis of triploids was characterized by the simultaneous occurrence of gonias and immature cytes together with mature gametes rather than overall retardation, though some triploids showed complete gonad maturation. Evidence of spawning was seen in both groups, and the quantity of germinal products emitted appeared to be similar in... |
| Tipo: Text |
Palavras-chave: Triploidy; Crassostrea gigas; Oyster; Gametogenesis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-4152.pdf |
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| Gerard, Andre; Peignon, Jean-marie; Chagot, Dominique. |
| Individual determination of ploidy level in experimentally induced triploid bivalve molluscs using image cytometry. The sexual maturation in bivalve molluscs causes decreased growth and sometimes increased mortalities. These drawbacks can be avoided by genetic sterilization (triploidy). Triploïdy induction require a egg treatment and a ploidy control at each generation. The ploidy level is usually determined by counting the chromosomes, a technique which is relatively easy for the trochophora stage and for juveniles measuring from 5 mm to 3 cm only. ln order to be able to follow up the evolution of the ploidy rate during any stage of animallife, we have developed an estimation method based on image cytometry. Individual DNA content is determined by... |
| Tipo: Text |
Palavras-chave: Image cytometry; Caryology; Triploidy; Bivalve; Imagerie numérique; Caryologie; Triploïdie; Bivalve. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-3202.pdf |
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| Suquet, Marc; Malo, Florent; Quere, Claudie; Ledu, Christophe; Le Grand, Jacqueline; Benabdelmouna, Abdellah. |
| Triploidy induction in shellfish aims to obtain faster growth and sterility of reared individuals. Triploid Pacific oysters are most often not totally sterile, but have greatly reduced reproductive capacities compared to those recorded in diploid individuals. The description of gamete output in triploid aquatic animals is most often confined to the assessment of fecundity in females. The present work aims at further describing gamete quality of triploid Pacific oysters and comparing it to values observed in diploid individuals. Diploid and triploid oysters (produced by mating tetraploid males × diploid females) were reared in standard conditions and then transferred to the wild to allow the offspring to attain reproductive maturity. At the end of... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Reproduction; Triploidy; Gamete. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00277/38835/37581.pdf |
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| Normand, Julien; Ernande, Bruno; Boudry, Pierre. |
| Today, triploidy is the most common method used to genetically improve marine bivalve aquaculture production. In the Pacific oyster Crassostrea gigas, triploidy affects many traits. The most proeminent one is reproductive effort, which is greatly reduced compared to diploids. The combined effects of physiological trade-offs between survival, growth and reproduction together with the reduction of reproductive effort in triploids explains the global enhancement of yield observed. However, the sterility of triploid oysters is only partial and some triploid individuals can show normal gametogenesis (i.e. similar to diploids), especially under warm conditions. In diploid oysters, the heritability of reproductive effort has been shown to be relatively low, but a... |
| Tipo: Text |
Palavras-chave: Parentage analysis; Triploidy; Oyster; Quantitative genetics; Gametogenesis. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/acte-4525.pdf |
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| Lapegue, Sylvie; Renault, Tristan. |
| The most known and certainly the most important contribution of genetics to the oyster indus try is the development of the triploid cupped oyster. Released for the first time in the 1980s, the triploid oyster has triplets of chromosomes instead of pairs. Currently, triploid oysters are obtained by crossing diploid and tetraploid oysters. These oysters are characterized by a near lack of milt in summer, which makes them generally popular with consumers, and by a faster growth, allowing a shorter production cycle. In parallel, the exploitation of natural resistance in the diversity of French oysters has been considered to improve the health of farmed populations which is a major challenge for the sustainability of aquaculture farms. Currently, the... |
| Tipo: Text |
Palavras-chave: Huître creuse; Triploïdie; Sélection; Maladies; Ostréiculture; Cupped oyster; Triploidy; Selection; Disease; Shellfish farming. |
| Ano: 2019 |
URL: https://archimer.ifremer.fr/doc/00498/60931/64326.pdf |
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| Gomes, Aline Dal`Olio; Vieira, Vanessa Aparecida Rocha Oliveira; Tabata, Yara Aiko; Takahashi, Neuza Sumico; Moreira, Renata Guimarães; Ribeiro, Cristiele Silva. |
| Triploid fish are usually sterile. Thus, the energy and nutrients intended for sexual maturation may be available to enhance flesh quality and physical growth. The present study aimed to investigate differences in the metabolic substrates, lipids and proteins, between storage tissues from diploid and triploid female rainbow trout. Monthly, metabolic substrates were quantified in liver, muscle, and ovaries, which were collected during the first reproductive cycle. In general, it was possible to identify a seasonal and similar deposition of metabolites in different tissues of 2n and 3n females, mainly at early stages of gonadal maturation. However, from the stages 5-6, the ovaries showed great differences between ploidies, with higher concentration of lipids... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Metabolism; Reproduction; Triploidy; Somatic indexes; Oncorhynchus mykiss.. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/44580 |
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| Piferrer, Francesc; Beaumont, Andy; Falguiere, Jean-claude; Flajshans, Martin; Haffray, Pierrick; Colombo, Lorenzo. |
| Polyploids can be defined as organisms with one or more additional chromosome sets with respect to the number most frequently found in nature for a given species. Triploids, organisms with three sets of homologous chromosomes, are found spontaneously in both wild and cultured populations and can be easily induced in many commercially relevant species of fish and shellfish. The major consequence of triploidy is gonadal sterility, which is of advantage in the aquaculture of molluscs since it can result in superior growth. In fish, the induction of triploidy is mainly used to avoid problems associated with sexual maturation such as lower growth rates, increased incidence of diseases and deterioration of the organoleptic properties. Triploidy can also be used... |
| Tipo: Text |
Palavras-chave: GMO; Transgenic containment; Genetic containment; Reproductive containment; Hybridisation; Sterility; Fish; Shellfish; Fish farming; Aquaculture; Tetraploidy; Triploidy; Polyploidy. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6648.pdf |
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| Gross,Maria Claudia; Schneider,Carlos Henrique; Matiello,Mara Cristina de Almeida; Leite,Maysa de Lima; Bertollo,Luiz Antonio Carlos; Artoni,Roberto Ferreira. |
| Morphological and chromosomal markers were used to infer the structure and genetic variability of a population of fish of the genus Astyanax, geographically isolated at sinkhole 2 of Vila Velha State Park, Paraná, Brazil. Two morphotypes types were observed, the standard phenotype I and phenotype II which showed an anatomical alteration probably due to an inbreeding process. Fluctuating asymmetry (FA) analysis of different characters showed low levels of morphological variation among the population from sinkhole 2 and in another population from the Tibagi river (Paraná, Brazil). The Astyanax karyotype was characterized in terms of chromosomal morphology, constitutive heterochromatin and nucleolar organizer regions. Males and females presented similar... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fish; Astyanax sp.; Fluctuating asymmetry; Karyotype structure; Triploidy; Genetic conservation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000400011 |
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| Peruzzi, Stefano; Chatain, Beatrice. |
| The optimal conditions for the retention of the second polar body in sea bass eggs were investigated by altering the timing, intensity and duration of application of pressure and cold shocks. Treatment optima for cold shocks were 0-1 degrees C for 15-20 min at 5 min after fertilisation (a.f.) and 8500 psi for 2 min at 6 min a.f. for pressure shocks. Meiogenesis was obtained by fertilising eggs with UV-irradiated homologous sperm (32,000 erg mm(-2)) and pressure or cold shocking eggs as above. 100% triploidy was induced following definition of liable periods for the disruption of the meiotic spindle obtained in gynogenesis. Ploidy investigations were performed on experimental groups by flow-cytometry. Verification of uniparental transmission in meiogens was... |
| Tipo: Text |
Palavras-chave: Parental variability; Pressure and cold shocks; Dicentrarchus labrax; Sea bass; Triploidy; Meiotic gynogenesis. |
| Ano: 2000 |
URL: http://archimer.ifremer.fr/doc/2000/publication-507.pdf |
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| Normand, Julien; Ernande, Bruno; Haure, Joel; Mccombie, Helen; Boudry, Pierre. |
| Early reproductive effort and growth were measured in 3 groups of the Pacific oyster Crassostrea gigas: 1 diploid group and 2 triploid groups resulting from either chemical induction (3nCB) or crosses between tetraploid and diploid parents (3nDT). Oysters were reared under intensive nursery conditions and sampled when 5 mo old. Reproductive effort was estimated by cross-sectional area measurements of the visceral mass (i.e. gonadic occupation) and maturation stage was assessed by qualitative histology. As expected, comparison of the reproductive patterns of these 3 groups revealed a lower reproductive effort in triploid individuals relative to diploid. However, gonadic occupation in triploid oysters was higher than expected in both 3nCB and 3nDT groups, as... |
| Tipo: Text |
Palavras-chave: Triploidy; Oyster; Gametogenesis; Crassostrea gigas. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6978.pdf |
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| De Decker, Sophie; Normand, Julien; Saulnier, Denis; Pernet, Fabrice; Castagnet, Sophie; Boudry, Pierre. |
| Several Vibrio species are known to be pathogenic to the Pacific oyster Crassostrea gigas. Survival varies according to pathogen exposure and high mortality events usually occur in summer during gametogenesis. In order to study the effects of gametogenetic status and ploidy (a factor known to affect reproduction allocation in oysters) on vibriosis survival, we conducted two successive experiments. Our results demonstrate that a common bath challenge with pathogenic Vibrio splendidus and Vibrio aestuarianus on a mixture of mature, spawning and non-mature oysters can lead to significant mortality. Previous bath challenges, which were done using only non-mature oysters, had not produced mortality. Immunohisto-chemical analyses showed the affinity of Vibrio... |
| Tipo: Text |
Palavras-chave: Experimental vibriosis; Crassostrea gigas; Vibrio; Survival distribution function; Triploidy; Reproductive effort. |
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00030/14108/11368.pdf |
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| Registros recuperados: 16 | |
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